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fluorogenic hdac substrate class 2 a  (BPS Bioscience)


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    BPS Bioscience fluorogenic hdac substrate class 2 a
    Fluorogenic Hdac Substrate Class 2 A, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hdac+substrate+2/Fluorogenic+HDAC+class+2a+substrate/pm41109068-446-37-43
    Average 94 stars, based on 21 article reviews
    fluorogenic hdac substrate class 2 a - by Bioz Stars, 2026-09
    94/100 stars

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    Inhibition of HDAC1 activity in HT29 cells after 24 h of incubation with (a) polyphenols in the presence of catalase (100 U/mL) or (b) methyleugenol and methyleugenol metabolites. HDAC activity was determined by the metabolic rate of a specific HDAC1 substrate. Recombinant HDAC1 was included as positive control (PC) and trichostatin A (TA) as a specific HDAC inhibitor. The data presented are mean ± SD of at least three independent experiments, each performed in a duplicate. The significances indicated were calculated in relation to the solvent control DMSO 0.5% v/v (Student's t -test: * P ≤ 0.05; ** P ≤ 0.01).

    Journal: Journal of Nutrition and Metabolism

    Article Title: Plant Polyphenols and Oxidative Metabolites of the Herbal Alkenylbenzene Methyleugenol Suppress Histone Deacetylase Activity in Human Colon Carcinoma Cells

    doi: 10.1155/2013/821082

    Figure Lengend Snippet: Inhibition of HDAC1 activity in HT29 cells after 24 h of incubation with (a) polyphenols in the presence of catalase (100 U/mL) or (b) methyleugenol and methyleugenol metabolites. HDAC activity was determined by the metabolic rate of a specific HDAC1 substrate. Recombinant HDAC1 was included as positive control (PC) and trichostatin A (TA) as a specific HDAC inhibitor. The data presented are mean ± SD of at least three independent experiments, each performed in a duplicate. The significances indicated were calculated in relation to the solvent control DMSO 0.5% v/v (Student's t -test: * P ≤ 0.05; ** P ≤ 0.01).

    Article Snippet: The HDAC reaction was initiated by adding 10 μ L of the diluted HDAC substrate (BOC- N ε -acetyl-L-lysine-7-amino-4-methylcoumarin) to each well.

    Techniques: Inhibition, Activity Assay, Incubation, Recombinant, Positive Control